Esterase Stain

Norm of Esterase Stain

Descriptive interpretation by hematologist.


Usage of Esterase Stain

Granulocytic sarcoma, leukemia.


Description of Esterase Stain

Stain of bone marrow to distinguish normal and leukemic cells of neutrophils, monocytes, and their precursors.


Professional Considerations of Esterase Stain

Consent form NOT required.

  1. Obtain glass slides, a lancet, a capillary tube, and a bone marrow tray.



  1. Obtain a bone marrow specimen and a fingerstick collection for peripheral blood smear.


Postprocedure Care

  1. Assess the bone marrow aspiration site for bleeding or hematoma.
  2. The client must lie flat for 1–2 hours after the procedure.
  3. Send the specimen slides to the laboratory immediately.


Client and Family Teaching

  1. Bone marrow aspiration is very painful but only for a brief moment.
  2. Results are normally available within 24 hours.


Factors That Affect Results

  1. Poor bone marrow sample will decrease the amount or quality of the cells, leading to inaccurate interpretation.


Other Data

  1. Staining of fresh specimens enhances assessment.
  2. See also Bone marrow aspiration analysis—Specimen for professional considerations related to the bone marrow aspiration procedure.
  3. Mitomycin-C may be a suitable agent for inhibiting RPE proliferation in vivo.