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Definition of Intervening sequence
Medically reviewed by Min Clinic Staff | Updated: January 2026
Intervening sequence: Part of a gene that is initially transcribed from the DNA into the primary RNA transcript but then is excised (removed) from it when the so-called exons sequences on either side of it are spliced together.
Intervening sequences, which are also called introns, are genetic sequences that intervene between the exons. The DNA of a gene is thereby made up of an alternation of introns and exons.