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Definition of Blot, Western
Medically reviewed by Dr. Otari Nergadze, Neurosurgeon | Updated: January 2026
Blot, Western: A technique in molecular biology that is used to separate and identify proteins. It is called a Western blot merely because it is similar to a Southern blot, which was named after its inventor, the British biologist M.E. Southern. For example, the Western blot assay method is commonly used to diagnose Lyme disease.