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Definition of Phosphorylation
Medically reviewed by Min Clinic Staff | Updated: January 2026
Phosphorylation: A biochemical process that involves the addition of phosphate to an organic compound. Examples include the addition of phosphate to glucose to produce glucose monophosphate and the addition of phosphate to adenosine diphosphate (ADP) to form adenosine triphosphate (ATP). Phosphorylation is carried out through the action of enzymes known as phosphotransferases or kinases.