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Definition of Spectrophotometer

Medically reviewed by Dr. Otari Nergadze, Neurosurgeon | Updated: January 2026

Spectrophotometer: A device which measures the amount of ultraviolet light absorbed by a substance. For example, vitamin A absorbs ultraviolet light so that a spectrophotometer can measure within minutes with almost perfect accuracy the amount of vitamin A in a given food. The spectrophotometer, a key instrument today in biomedical laboratories, was invented in 1939 by the American chemist Arnold O. Beckman (1900-2004).