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Definition of Nitrogenous base

Medically reviewed by Min Clinic Staff | Updated: January 2026

Nitrogenous base: A molecule that contains nitrogen and has the chemical properties of a base. The nitrogenous bases in DNA are adenine (A), guanine (G), thymine (T), and cytosine (C). The nitrogenous bases in RNA are the same, with one exception: adenine (A), guanine (G), uracil (U), and cytosine (C).